Publications
Full list of published work:
2024
Takagishi M, Yue Y, Gray RS, Verhey KJ, and Wallingford JB. (2024). Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells. Disease Models & Mechanisms 17(2):dmm050137. PMID: 38235522. PMCID: PMC10924229.
Waas B, Carpenter BS, Franks NE, Merchant OQ, Verhey KJ, and Allen BL. (2024). Dual and opposing roles for the kinesin-2 motor, KIF17, in Hedgehog-dependent cerebellar development. Science Advances 10(17):eade1650. PMID: 38669326. PMCID: PMC11051677.
Geng Q, Keya JJ, Hotta T, and Verhey KJ. (2024). The kinesin-3 KIF1C undergoes liquid-liquid phase separation for accumulation of specific transcripts at the cell periphery. EMBO Journal Jun 19. Epub ahead of print. PMID: 38898313
Kim D, Cianfrocco MA, Verhey KJ, and Smith GA. (2024). The HSV-1 pUL37 protein promotes cell invasion by regulating the kinesin-1 motor. Proc Natl Acad Sci U S A.121(19):e2401341121. PMID: 38696466
2023
Hotta T, Plemmons A, Gebbie M, Ziehm TA, Blasius TL, Johnson C, Verhey KJ, Pearring JN, and Ohi R. (2023) Mechanistic Analysis of CCP1 in Generating ΔC2 α-Tubulin in Mammalian Cells and Photoreceptor Neurons. Biomolecules 13(2):357. PMID: 36830726. PMCID: PMC9952995.
Tan Z, Yue Y, da Veiga Leprevost F, Haynes SE, Basrur V, Nesvizhskii A, Verhey KJ, and Cianfrocco MA. (2023). Autoinhibited kinesin-1 adopts a hierarchical folding pattern. eLife 12:RP86776. PMID: 37910016. PMCID: PMC10619981
Birk MA, Liscovitch-Brauer N, Dominguez MJ, McNeme S, Yue Y, Hoff JD, Twersky I, Verhey KJ, Sutton RB, Eisenberg E, and Rosenthal JJC. (2023). Temperature-dependent RNA editing in octopus extensively recodes the neural proteome. Cell 186(12):2544-2555.e13. PMID: 37295402. PMCID: PMC10445230
Minckley, TF, Salvagio, LA, Fudge, DH, Verhey, KJ, Markus, SM, and Qin, Y (2023). Zn2+ decoration of microtubules arrests axonal transport and displaces tau, doublecortin, and MAP2C. The Journal of Cell Biology 222(8):e202208121. PMID: 37326602.PMCID: PMC10276529
Yue Y, Hotta T, Higaki T, Verhey KJ, and Ohi R. (2023). Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice. Current Biology 33(19):4111-4123.e7. PMID: 37716348. PMCID: PMC10592207
2022
Yue Y, Engelke MF, Blasius TL, and Verhey KJ. (2022). Hedgehog-induced ciliary trafficking of kinesin-4 motor KIF7 requires intraflagellar transport but not KIF7’s microtubule binding. Molecular Biology of the Cell 33(1):br1. PMID: 34705483.PMCID: PMC8886809
Konjikusic MJ, Lee C, Yue Y, Shrestha BD, Nguimtsop AM, Horani A, Brody A, Prakash VN, Gray RS, Verhey KJ, and Wallingford JB. (2022). Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes. Journal of Cell Science 136(5): jcs.259535. PMID: 35531639. PMCID: PMC9357393
Balseiro-Gómez S*, Park J*, Yue Y, Ding C, Shao L, Ҫetinkaya S, Kuzoian C, Hammarlund M, Verhey KJ, and Yogev S. (2022). Neurexin and Frizzled intercept axonal-transport at microtubule minus-ends to control synapse formation. Developmental Cell 57(15):1802-1816. PMID: 35809561. PMCID: PMC9378695
Budaitis BG, Badieyan S*, Yue Y*, Blasius TL, Reinemann DN, Lang MJ, Cianfrocco MA, and Verhey KJ. (2022). A kinesin-1 variant reveals motor-induced microtubule damage in cells. Current Biology 32(11):2416-2429. PMID: 35504282. PMCID: PMC9993403
Hotta T, McAlear T, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, and Ohi R. (2022). EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Current Biology 32(18):3898-3910.e14. PMID: 35963242. PMCID: PMC9530018
Poulos A, Budaitis BG, and Verhey KJ. (2022). Single-motor and multi-motor motility properties of kinesin-6 family members. Biology Open 11(10):bio059533. PMID: 36178151. PMCID: PMC9581516
2021
Hotta T, Haynes SE, Blasius TL, Gebbie M, Eberhard EL, Sept D, Cianfrocco M, Verhey KJ, Nesvizhskii AI, and Ohi R. (2021). Parthenolide destabilizes microtubules by covalently modifying tubulin. Current Biology 31(4):900-907.e6. PMID: 33482110. PMCID: PMC7931505
*Budaitis BG, *Jariwala S, *Rao L, Yue Y, Sept D, Verhey KJ, and Gennerich A. (2021). Pathogenic Mutations in the Kinesin-3 Motor KIF1A Diminish Force Generation and Movement Through Allosteric Mechanisms. Journal of Cell Biology 220(4):e202004227. PMID: 33496723. PMCID: PMC7844421
Varela PF, Chenon M, Velours C, Verhey KJ, Ménétrey J, and Gigant B. (2021). Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism. FEBS Open Biology 11(3):564-577. PMID: 33513284. PMCID: PMC7931232
Kearns S. Mason FM, Rathmell WK, Park IY, Walker CL, Verhey KJ, and Cianfrocco MA. (2021). Molecular determinants for α-tubulin methylation by SETD2. Journal of Biological Chemistry 297(1):100898. PMCID: PMC8294582
Karki M, Jangid RK, Anish R, Seervai RNH, Bertocchio JP, Hotta T, Msaouel P, Jung SY, Grimm SL, Coarfa C, Weissman BE, Ohi R, Verhey KJ, Hodges HC, Burggren W, Dere R, Park IY, Prasad BVV, Rathmell WK, Walker CL, and Tripathi DN. (2021). A cytoskeletal function for PBRM1 reading methylated microtubules. Science Advances 7(14):eabf2866. PMID: 33811077. PMCID: PMC11059954
Koenning M, Wang X, Karki M, Jangid RK, Kearns S, Tripathi DN, Cianfrocco M, Verhey KJ, Jung SY, Coarfa C, Ward CS, Kalish BT, Grimm SL, Rathmell WK, Mostany R, Dere R, Rasband MN, Walker CL, and Park IY. (2021). Neuronal SETD2 activity links microtubule methylation to an anxiety-like phenotype in mice. Brain 144(8):2527-2540. PMID: 34014281. PMCID: PMC8418347
Blasius TL*, Yue Y*, Prasad R, Liu X, Gennerich A, and Verhey KJ. (2021). Sequences in the stalk domain regulate autoinhibition and ciliary tip localization of the immotile kinesin-4 KIF7. Journal of Cell Science 134(13):jcs258464. PMID: 34114033. PMCID: PMC8277141
2020
Gan KJ, Akram A, Blasius TL, Ramser EM, Budaitis BG, Gabrych DR, Verhey KJ, and Silverman MA. (2020). GSK3β Impairs KIF1A Transport in a Cellular Model of Alzheimer’s Disease but Does Not Regulate Motor Motility at S402. eNeuro 7(6):ENEURO.0176-20.2020. PMID: 33067366. PMCID: PMC7768277
Seervai RNH, Jangid RK, Karki M, Tripathi DN, Jung SY, Kearns SE, Verhey KJ, Cianfrocco MA, Millis BA, Tyska MJ, Mason FM, Rathmell WK, Park IY, Dere R, and Walker CL. (2020) .The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase. Science Advances 6(40):eabb7854. PMID: 33008892.PMCID: PMC7852384
Kaur S, Van Bergen NJ, Verhey KJ, Nowell CJ, Budaitis B, Yue Y, Ellaway C, Brunetti-Pierri N, Cappuccio G, Bruno I, Boyle L, Nigro V, Torella A, Roscioli T, Cowley MJ, Massey S, Sonawane R, Burton MD, Schonewolf-Greulich B, Tümer Z, Chung WK, Gold WA, and Christodoulou J. (2020) Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A). Human Mutation 41:1761-1774. PMID: 32652677.PMCID: PMC7908811
Spriggs CC, Badieyan S, Verhey KJ, Cianfrocco MA, and Tsai B. (2020) Golgi-associated BICD adaptors couple ER membrane penetration and disassembly of a viral cargo. Journal of Cell Biology 219(5):e201908099. PMID: 32259203 PMCID: PMC7199864
2019
Arpağ G, Norris SR, Mousavi SI, Soppina V, Verhey KJ, Hancock WO, and Tüzel E. (2019). Motor dynamics underlying cargo transport by pairs of kinesin-1 and kinesin-3 motors. Biophysical Journal. 116(6):1115-1126. PMID: 30824116. PMCID: PMC6428962
Schimert KI, Budaitis BG, Reinemann DN, Lang MJ, and Verhey KJ. (2019). Intracellular cargo transport by single-headed kinesin motors. Proceedings of the National Academy of Sciences USA 116(13):6152-6161. PMID: 30850543. PMCID: PMC6442636
Engelke MF, Waas B, Kearns SE, Suber A, Boss A, Allen BL, and Verhey KJ. (2019). Acute inhibition of heterotrimeric kinesin-2 function reveals mechanisms of intraflagellar transport in mammalian cilia. Current Biology 29(7):1137-1148. PMID: 30905605. PMCID: PMC6445692
Lessard DV, Zinder OJ, Hotta T, Verhey KJ, Ohi R, and Berger CL. (2019). Regulation of KIF1A motility via polyglutamylation of tubulin C-terminal tails. Journal of Biological Chemistry 294(16):6353-6363. PMID: 30770469. PMCID: PMC6484136
Budaitis BG*, Jariwala S*, Reinemann DN*, Schimert KI, Scarabelli G, Grant BJ, Sept D, Lang MJ, and Verhey KJ. (2019). Neck linker docking is critical for Kinesin-1 force generation in cells but at a cost to motor speed and processivity. eLife 8: e44146. PMID: 31084716. PMCID: PMC6533058
Blasius TL, Takao D, and Verhey KJ. (2019). NPHP proteins are binding partners of nucleoporins at the base of the primary cilium. PLoS One 14(9):e0222924. PMID: 31553752. PMCID: PMC6760808
2018
Yue Y, Blasius TL, Zhang S, Jariwala S, Walker B, Grant BJ, Cochran JC, and Verhey KJ. (2018). Altered chemomechanical coupling causes impaired motility of the kinesin-4 motors KIF27 and KIF7. Journal of Cell Biology 217(4):1319-1334. PMID: 29351996. PMCID: PMC5881503
Ravindran MS, Spriggs CC, Verhey KJ, and Tsai, B. (2018). Dynein engages and disassembles cytosol-localized SV40 to promote infection. Journal of Virology 92: e00353-18. PMID: 29593037. PMCID: PMC5974495
Kelliher MT, Yue Y, Ng A, Kamiyama D, Huang B, Verhey KJ, and Wildonger J. (2018). Regulated autoinhibition of kinesin-1 is essential to polarized dendritic transport. Journal of Cell Biology 217(7):2531-2547. PMID: 29728423. PMCID: PMC6028532
Tymanskyj SR, Yang BH, Verhey KJ, and Ma L. (2018). MAP7 Regulates Axon Morphogenesis by Recruiting Kinesin-1 to Microtubules and Modulating Organelle Transport. eLife 7: e36374. PMID: 30132755. PMCID: PMC6133550
2017
Breznau EB, Murt M, Blasius TL, Verhey KJ, and Miller AL. (2017). The MgcRacGAP SxIP motif tethers Centralsindlin to microtubule plus ends in Xenopus laevis. Journal of Cell Science 130(10):1809-1821. PMID: 28389580. PMCID: PMC5450188
Ravindran MS, Engelke M, Verhey KJ and Tsai B. (2017). Exploiting the kinesin-1 molecular motor to generate a virus membrane penetration site. Nature Communications 8:15496. PMID: 28537258. PMCID: PMC5458101
Takao D, Wang L, Boss A, and Verhey KJ. (2017). Protein interaction analysis provides a map of the spatial and temporal organization of the ciliary gating zone. Current Biology 27(15):2296-2306. PMID: 28736169. PMCID: PMC5576555
Luo W, Ruba A, Takao D, Zweifel LP, Zweifu LP, Lim RYH, Verhey KJ, and Yang W. (2017). Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium. Scientific Reports 7:15793. PMID: 29150645. PMCID: PMC5693955
2016
Engelke MF, Winding M, Yue Y, Shastry S, Teloni F, Reddy S, Blasius TL, Soppina P, Hancock WO, Gelfand VI, and Verhey KJ. (2016). Engineered kinesin motor proteins amenable to small molecule inhibition. Nature Communications 7:11159. PMID: 27045608. PMCID: PMC4822052
Padzik A, Deshpande P, Hollos P, Franker M, Rannikko EH, Cai D, Prus P, Mågård M, Westerlund N, Verhey KJ, James P, Hoogenraad C, and Coffey ET. (2016). KIF5C S176 Phosphorylation Regulates Microtubule Binding and Transport Efficiency in Mammalian Neurons. Frontiers in Cellular Neuroscience 10:57. PMID: 27013971. PMCID: PMC4791394
Park IY, Powell RT, Tripathi DN, Dere R, Ho TH, Blasius TL, Chiang Y-C, Davis IJ, Fahey CC, Hacker KE, Verhey KJ, Bedford MT, Jonasch E, Rathmell WK, and Walker CL. (2016). Dual Chromatin and Cytoskeletal Remodeling by SETD2. Cell 166, 950–962. PMID: 27518565. PMCID: PMC5101839
2015
Carpenter BS, Barry RL, Verhey KJ, and Allen BL. (2015). The heterotrimeric kinesin-2 complex interacts with and regulates GLI protein function. Journal of Cell Science 128(5): 1034-1050. PMID: 25588831. PMCID: PMC4342584
Norris SR, Nunez MR, and Verhey KJ. (2015). Influence of fluorescent tag on the motility properties of kinesin-1 in single-molecule assays. Biophysical Journal 108(5): 1133-1143. PMID: 25762325. PMCID: PMC4375428
Scarabelli G*, Soppina V*, Yao XQ, Atherton J, Moores CA, Verhey KJ, and Grant BJ. (2015). Mapping the Processivity Determinants of the Kinesin-3 Motor Domain. Biophysical Journal 109(8):1537-1540. PMID: 26488644. PMCID: PMC4624112
2014
Soppina V, Norris SR, Dizaji A, Kortus M, VeatchS, PeckhamM, and Verhey KJ. (2014). Dimerization of mammalian kinesin-3 motors results in super processive motion. Proceedings of the National Academy of Sciences USA 111(15): 5562-5567. PMID: 24706892. PMCID: PMC3992690
Kaul N, Soppina V, and Verhey KJ. (2014). Effects of a-tubulin K40-acetylation and detyrosination on kinesin-1 motility in a purified system. Biophysical Journal 106(12): 2636-2643. PMID: 24940781. PMCID: PMC4070028
Soppina V and Verhey KJ. (2014). The family-specific K-loop influences the microtubule on-rate but not the processivity of kinesin-3 motors. Molecular Biology of the Cell 25(14): 2161-2170. PMID: 24850887. PMCID: PMC4091829
Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, and Verhey KJ. (2014). A method for multiprotein assembly in cells reveals independent action of kinesins in complex. Journal of Cell Biology 207(3): 393-406. PMID: 25365993. PMCID: PMC4226728
Takao D, Dishinger JF, Kee HL, Pinskey JM, Allen BL, and Verhey KJ. (2014). An assay for clogging the ciliary pore complex distinguishes mechanisms of cytosolic and membrane protein entry. Current Biology 24(19): 2288-2294. PMID: 25264252. PMCID: PMC4189977
Broekhuis JR, Verhey KJ, and Jansen G. (2014). Regulation of cilium length and intraflagellar transport by the RCK-kinases ICK and MOK in renal epithelial cells. PLoS One 9(9): e108470. PMID: 25243405. PMCID: PMC4171540
Williams CL, McIntyre JC, Norris SR, Jenkins PM, Zhang L, Pei Q, Verhey KJ, and Martens JR. (2014). Direct evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia. Nature Communications 5: 5813-5825. PMID: 25504142. PMCID: PMC4284812
2013
Blasius TL, Reed N, Slepchenko BM, and Verhey KJ. (2013). Recycling of kinesin-1 motors by diffusion after transport. PLoS One 8(9): e76081. PMID: 24098765. PMCID: PMC3786890
2012
Soppina V*, Herbstman JF*, Skiniotis G, and Verhey KJ. (2012). Luminal Localization of a-tubulin Acetylation by Cryo-EM Analysis of Fab-labeled Microtubules. PLoS One 7(10): e48204. PMID: 23110214. PMCID: PMC3482196
Kee HL, Dishinger JF, Blasius TL, Liu CJ, Margolis B, and Verhey KJ. (2012). A size-exclusion barrier and nucleoporins characterize a ciliary pore complex that regulates transport into cilia. Nature Cell Biology 14(4): 431-437. PMID: 22388888. PMCID: PMC3319646
2011
Fan S, Whiteman EL, Hurd TW, McIntyre JC, Dishinger JF, Liu CJ, Martens JR, Verhey KJ, Sajjan U, and Margolis BL. (2011). Induction of Ran GTP Drives Ciliogenesis. Molecular Biology of the Cell 22(23): 4539-4548. PMID 21998203. PMCID: PMC3226473
2010
Friedman JR, Webster BM, Mastronarde DN, Verhey KJ and Voeltz GK. (2010). ER dynamics and ER-mitochondrial contacts occur on acetylated microtubules. Journal of Cell Biology 190: 363-375. PMID: 20696706. PMCID: PMC2922647
Dishinger JF, Kee HL, Jenkins PM, Fan S, Hurd TW, Hammond JW, Truong YNT, Margolis B, Martens JR, and Verhey KJ. (2010). Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-b2 and Ran-GTP. Nature Cell Biology 12: 703-710. PMID: 20526328. PMCID: PMC2896429
Hammond JW, Blasius TL, Soppina V, Cai D, and Verhey KJ. (2010). Autoinhibition of the kinesin-2 motor KIF17 via dual intramolecular mechanisms. Journal of Cell Biology 189: 1013-1025. PMID: 20530208. PMCID: PMC2886353
Hammond JW*, Huang C-F *, Kaech S, Jacobson C, Banker G, and Verhey KJ. (2010). Posttranslational modifications of tubulin and the polarized transport of Kinesin-1 in neurons. Molecular Biology of the Cell 21:572-83. PMID: 20032309. PMCID: PMC2820422
2009
Qian M*, Cai D*, Verhey KJ, and Tsai B. (2009). A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection. PLoS Pathogen 5:e1000465. PMID: 19503604. PMCID: PMC2685006
Cai D, McEwen D, Martens JR, Meyhofer E, and Verhey KJ. (2009). Single Molecule Imaging Reveals Differences in Microtubule Track Selection by Kinesin Motors. PLoS Biology 7: e1000216. PMID: 19823565. PMCID: PMC2749942
Hammond JW, Cai D, Blasius TL, Li Z, Jiang Y, Jih G, Meyhofer E, and Verhey KJ. (2009). Mammalian Kinesin-3 motors are dimeric in vivo and move by processive motility upon release of autoinhibition PLoS Biology 7: e72. PMID: 19338388. PMCID: PMC2661964
2008
Hammond JW, Griffin K, Jih GT, Stuckey J, and Verhey KJ. (2008). Cooperative versus Independent transport of Kinesin-1 cargoes. Traffic 9: 725-741. PMID: 18266909.
2007
Cai D, Verhey KJ, and Meyhofer E. (2007). Tracking Single Kinesin Molecules in the Cytoplasm of Mammalian Cells. Biophysical Journal 92: 4137-4144. PMID: 17400704. PMCID: PMC1877757
Cai D, Hoppe AD, Swanson JA, and Verhey KJ. (2007). Kinesin-1 Structural Organization and Conformational Changes Revealed by FRET Stoichiometry in Live Cells. Journal of Cell Biology 176: 51-63. PMID: 17200416. PMCID: PMC2063625
Blasius TL, Cai D, Jih GT, Toret CP, and Verhey KJ. (2007). Two binding partners cooperate to activate the molecular motor Kinesin-1. Journal of Cell Biology 176: 11-17. PMID: 17200414. PMCID: PMC2063617
2006
Reed NA, Cai D, Blasius TL, Jih GT, Meyhofer E, Gaertig J, and Verhey KJ. (2006). Microtubule acetylation promotes Kinesin-1 binding and transport. Current Biology 16: 2166-2172. PMID: 17084703.
Jenkins PM, Hurd TW, Zhang L, McEwen DP, Brown RL, Margolis B, Verhey KJ, and Martens JR. (2006). Ciliary targeting of olfactory CNG channels requires the CNGB1b subunit and the kinesin-2 motor protein KIF17. Current Biology 16: 1211-1216. PMID: 16782012.